Effect of Aldehyde-Quaternary Ammonium Compound Based Disinfectant on Hatching Parameters of Embryonated Chicken Egg Contaminated with E. Coli

Abstract

 E .coli is one of the most important pathogens that causes embryonic death and reduces hatchability that causing severe economic losses in poultry production. E. coli is resistant to some commonly used antibiotics in poultry, so we must have other approaches to control E. coli. The aim of this study is to investigate the effectiveness of glutaraldehyde (ALDEKOL GDA) disinfectant on E. coli O78
experimentally contaminating specific pathogen free embryonated chicken egg (SPF ECE) eggshell. In this study apply the antibiotic susceptibility of the identified isolate to know antibiotic E coli resistance. 150 fertile SPF embryonated chicken egg were divided into five equal groups (30 eggs per each group) Group number (1) be non-infected non-treated (control negative), while group number (2)
considered non-infected treated (treatment control). Group (3) infected non-treated group, and those of groups 4 and 5 were direct wet spray disinfected using (Aldekol GDA®) one time for group (4), while group (5) was re-sprayed twice. All egg groups were candled for dead in shell embryos which were subjected to bacterial examination for reisolation of E. coli from egg shell, liver and yolk sac.
Microbiological examination was carried out on live and dead in egg shell, liver and yolk sac. The obtained results, Resistance were detected to 17 of the 26 antibiotics commonly used in poultry. Results demonstrated that total bacterial counts were reduced as a result of treatment with Aldehyde – QAC based disinfectant. infected group 5 (infected and disinfected twice) showed highest hatchability (93.3%) followed by group 4 (infected and disinfected once) which showed 73.3% hatchability while group 3 (infected non treated group) showed the lowest hatchability (10%), group 2 hatchability was not affected. disinfection power increased against E .coli O78 when using disinfection more than one time. Reisolation of E. coli from group 3 egg shell, yolk sac and liver was the highest followed by group 4 that treated with disinfectant once while group 5 was the lowest. Conclusively ALDEKOL GDA disinfectant displays a high rate of hatchability and reduces embryonic mortalities and production of high quality chicks. Hatchability improved when ALDEKOL GDA disinfectant resprayed twice. ALDEKOL GDA was effective disinfectant, as reduce total bacterial counts and
eliminate E. coli from fertile egg.