Ameliorative Effect of Terminalia muelleri Leaves Extract on Kidney Tissue in Streptozotocin – Induced Diabetic Rats
Keywords:
Diabetes, kidney disorder, inflammation, oxidative stress, antioxidant, amyloid protein, Terminalia mulleriAbstract
The current study aimed to explore the potential renal-protective effect of Terminalia Muelleri leaves extract (TME) in streptozotocin (STZ)–induced diabetic rats. Experimental diabetes mellitus was created by subcutaneous injection of STZ at initial dose (27.5mg/kg), followed by three successive doses (11.25 mg/kg). Following the induction of diabetes, the diabetic rats were divided into three groups as the following, STZ group, two groups of diabetic rats treated with a daily oral dose of TME (200mg /kg b.wt) or Pioglitazone (PIO) drug (1.58mg /kg b.wt) for four weeks (STZ+ TME and STZ + PIO. In the same time, two groups of healthy rats were employed the first group was conducted as control non treated group and the rats of the second one were administered a daily oral dose of TME
(200mg /kg b.wt., TME group) for four weeks. The induction of kidney injury in diabetic rats was manifested by the recorded changes in kidney function-related parameters in serum; creatinine, urea, total protein and albumin, cystatin C (CysC), kidney injury molecule-1 (KIM-1), oxidative stress and antioxidant biomarkers (MDA level, GSH content and CAT activity) in renal tissue, with the assessment
of inflammatory– associated biomarkers ((NF-κB, TNF-α and IL-1β) in renal tissue. Pathological and histochemical examination of renal tissue of diabetic rats revealed many histological changes with significant formation of amyloid positive protein with decreasing of total protein content. The obtained results revealed that, TME or PIO administration significantly ameliorated kidney injury with the
tendency to lower the incidence of histological and changes in renal tissue of diabetic rats. The obtained results showed that, TME elicited a pronounced improving effect in comparison with PIO that was used as a reference antidiabetic drug. In conclusion: Our results suggested that TME could be a better alternative therapy in ameliorating diabetic-associated kidney disorders.
