Micropropagation of Schefflera amate Plants
Keywords:Schefflera amate, Araliaceae, Micropropagation, in vitro, in vivo, Acclimatization
Schefflera Amate an evergreen ornamental plant native of Australia and New Guinea which is one of the largest plant groups in the family Araliaceae. The aim of this study is to develop an efficient protocol for the rapid in vitro micropropagation of Schefflera Amate Plants via enhanced axillary bud proliferation from single nodal explants cultured on full strength MS basal medium, fortified with 30g/l sucrose, 4g/l gelrite augmented with diverse concentrations of phytohormones as BA, NAA, IBA and their combinations. In general, the prevailing observe concluded that the height effects for initiation level turned into recorded on MS medium fortified with NAA and BA at 1.00 and 0.50 mg/l, respectively. Meanwhile, supplemented medium with BA and NAA at 4.00 and 1.00 mg/l, respectively, gave upward push to the height effects for multiplication level. Regarding rooting level, the height effects have been recorded whilst the neoformed shoots of multiplication level have been divided singly and cultured on MS medium plus IBA and NAA at 2.00 and 0.250 mg/l, respectively which led to the maximum mean number of roots formed per propagule. Neoformed plantlets had been acclimatized ex vitro and in vivo vigorously in combination of perlite and peatmoss at (1:1) or (2: 1) and (2: 2) consecutively, further to fixed volume (1 portion) of sterile sand; which resulted in the highest mean value of survival percentage/plant (100%) and showed true-to-type plants ex vitro.
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