Effects of Grazing Dairy Cows on Bt and Non-Bt Cotton Crop Residues on the relationship between Rumen Total Bacterial Count and the proportion of Oleic acid in milk in Gezira State, Sudan
Keywords:Grazing, Dairy Cows, Bt and Non-Bt Cotton Crop Residues, total bacteria rumen fluid, milk Oleic acid, correlation between total bacteria rumen fluid and milk Oleic acid
The objective of this experiment was to the relationship between rumen total bacteria count and milk Oleic (g\100g) acid. Milk from cows grazed on Bt and non-Bt cotton crop residues (CCR). Cows were milked once a day by employing full hand milking, put in individual bottle, weigh, recorded, after weigh each group was collected and stored in separate tanks. ( Bt and non- Bt) Immediately after reaching the tank, it was cooled labeled around 3°C. Milk sample from each cow was collected at week 0 (Before introduction of animals for grazing), 1, 2, 3, 4 and 5 during the 5 weeks of experimental feeding. The collected milk was frozen and then transferred to the University of Gezira Laboratories for chemical analysis. Fatty acids analyzed determine by GC. Approximately 250 mL rumen fluid sample from each cow was collected on weeks 0 (Before introduction of animals for grazing), 1, 2, 3, 4 and 5 of the grazing period via stomach tubing. They were acidified with either 200 μL of 50% (volume/volume) sulfuric acid or 2 mL of 25% (weight/volume) meta phosphoric acid and stored at −20°C until later analyses of total bacterial count. The results revealed the rumen total bacteria, Rumunococus flavefaciens, Fibrobacter succinogens, Methanogenes from grazing on Bt CCR were negatively correlated with the milk Oleic acid. While rumen Rumunococus albus, from grazing on Bt and Non- CCR were negatively correlated with the milk Oleic acid.
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