Molecular Identification of Some Endophytic Bacteria Isolated from Leaves and Roots of Some Cultivars of Date Palm (Phoenix Dactylifera) and Its Anti-Fungal Activity Against Macrophomina Phaseolina

Abstract

Date palm tree (the blessed tree) considers a green treasure cause of its rich of nutrients carbohydrates, vitamins, and minerals, etc. Unfortunately, many bacterial and fungal diseases infected palm. Charcoal rot disease is one of the economic importance disease which caused by Macrophomina phaseolina. Fifty endophytic bacteria in both of leaves and roots in several cultivars of date palm were isolated and tested in vitro against M.phaseolina. Ten of the isolated bacteria got effect on growth of the fungus and these bacteria were identified as Brevundimonas diminuta, Streptomyces murinus, Enterobacter cloacae subsp. Dissolven, Brucella intermedia, Sphingobacterium nematocida, Sphingobacterium spiritivorum, Sphingobacterium spiritivorum, Sphingobacterium tabacisoli, Sphingobacterium soli, and Brucella lupine. Antagonistic In vivo using 10 bacteria versus M.phaseolina was carried out in green-house and the best results after 45 and 90 days were using Strept.murinus. many biochemical were estimated in In vivo experiment and the results showed total indol were high in infected plants, total phenol were high in Sph.spiritivorum strain AR2, and in pigments the high chlolrphyll a and b and carotenoids were in Strept.murinus, Brucella intermedia,and Sphingobacterium tabacisoli respectively