Genetic Basis for Salt Stress Tolerance in Wheat through Using ISSR Markers

Abstract

This study was launched as a serious attempt to genetically improve in wheat crop with the aim of increasing its tolerance to salt stress under Egyptian conditions. The degrees of negative risks of salt stress began to increase over the past few years, which led to a reduction in agricultural areas for growing strategic crops, especially wheat. On this basis, it was necessary to make a scientific leap in
the field of plant breeding and biotechnology with the aim of obtaining new wheat lines that are tolerant to dangerous environmental factor, besides, its high yields. Some promising wheat accessions included (Parents & Hybrids) were tested under normal and salinity conditions through evaluating agromorphological, biochemical, salinity tolerance indices and physiological attributes related to salinity
tolerance in this regard. Further, eleven ISSR primers were used to generate all genetically molecular differences among all wheat entries which confirmed highly tolerance and yielding. The final results obtained highly yield and salinity tolerance in five wheat hybrids besides, their parents depending on all data calculated in both experiments. Molecular markers showed polymorphism % closed to 100.0% among all wheat accessions using SR-08 and SR-45 primers besides, 34 unique bands.