In vitro Multiple Shoot Induction under Micropropagation Condition of Cocculus laurifolius (L.)

Abstract

In vitro trials were intended to find out a well protocol for micropropagation of Cocculus laurifolius plant. Shoots tips were effectively surface sterilized with 1.5 % NaOCl for 20 min which gave the best percentage of survival explant and free contamination. Shoot tip explants of the plants cultured on MS medium at full strength with activated charcoal to give the highest shoot length and number of leaves. For shoot formation, BA was better than Kin to produce the highest number of shoots/shoot. For rooting stage, 2.0 mg/l NAA was better than IBA to establish rooted shoots on full MS medium. Plantlets grew with a healthy appearance and the best percentage of acclimatization (81.67%) was observed when plantlets cultured in a peatmoss under plastic tunnel under plastic house conditions. RAPD-PCR (Randomly Amplified Polymorphic DNA) results confirmed the role of fragments polymorphism of micropropagated plants as compared to mother plants (in vivo) when using different growth regulators micropropagated Cocculus plants during tissue culture technique.