Response of Shoot Multiplication and Characteristics of Chinese Mandarin as Affected by Modification of MS Medium

Abstract

Micro scions of Citrus which are formed in vitro are very important in virus-free plants production. Micro scion multiplication faces multi obstacles such as drop leaves and shoots tip necrosis. So, this investigation aims to develop the micro propagation medium through examine the effect of modification of the mineral's concentrations in medium of chinese mandarin. Explants were cultured on full, half and quarter strength of MS medium individual or in combination with different concentrations (25, 50 and 100% of recommended rate) of MgSO4, 7H2O, MnSO4, 4H2O, CaCl2, 2H2O and KH2PO4. All media supplemented with 2 mg/l BA. The highest axillary shoot number (13 shoots/ jar) was obtained from full MS strength medium supplemented with 25% of MgSO4, 7H2O, MnSO4. 4H2O, CaCl2, 2H2O and KH2PO4. While, full MS strength supplemented with 50% of MgSO4. 7H2O and KH2PO4 recorded the highest value (55shoots/jar) of axillary shoots. On contrary, the highest number of adventitious shoots (65shoots/jar) resulted from full MS strength supplemented with 50% of MgSO4, 7H2O and CaCl2, 2H2O. Interestingly, full MS strength supplemented with 50% of MgSO4, 7H2O, MnSO4, 4H2O and KH2PO4 or supplemented with 25% of MgSO4, 7H2O, MnSO4. 4H2O, CaCl2, 2H2O and KH2PO4 prevent drop leaves phenomenon and shoot tip necrosis as compared to control treatment.