Improvement of in vitro production of mandarin microscions and enhancement success of in vivo micrografting
Keywords:Micrografting, PP333, ABA, IAA, mandarin, citrus
Traditional propagation method of citrus is a main cause of virus transportation from the infected trees through scions. So, the aim of this work is improvement the in vitro production and hardening of micro scions of mandarin. As well as, employment of the micro-scions to be successfully grafted on rootstock grown in greenhouse, as a new commercial method, through examine the effect of growth retardant and auxin on grafting successful percent of in vitro produced micro-scions in greenhouse. In this study, micro-scions of chinese mandarin were resulted from multiplication stage after cultured it on MS medium supplemented with different growth retardant types and concentrations as paclopetrazol (PP333) at different concentrations 0.5, 0.6, and 0.7mg/l and absacic acid (ABA) at different concentrations 2.5, 5.0 and 7.5μg/l for one month before be used as scions in greenhouse. After harvesting of micro-scions, it transferred to greenhouse and dipped in 10 mg/l IBA for different duration (0, 5, 10 and 15 min) prior to grafting process in greenhouse. After 60 days from grafting, scions derived from MS medium supplemented with 0.5 mg/l PP333 combined with dipping in IBA for 15 min, scions derived from MS medium supplemented with 0.7 mg/l PP333 combined with dipping in IBA for10 min,
scions derived from MS medium supplemented with 5.0 μg/l ABA combined with dipping in IBA for 10 min, scions derived from MS medium supplemented with 7.5 μg/l ABA without dipping in IBA and scions derived from MS medium supplemented with 7.5 μg/l ABA combined with dipping in IBA for 10 min) maximized successful grafts percentage (100 %) compared with control (40%). Growth
parameters were superior after 50 and 80 days of grafting as a result of the most treatments compared with control.
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