Antibacterial Efficacy of Photoactivated Irrigation of Diode and Photon-Induced Photoacoustic Streaming (Pips) Using Er-Yag Lasers On Infected Teeth

Authors

  • Nancy A. A. ElShafei Restorative and Dental Materials Department, Oral and Dental Research Institute , National Research Centre, Cairo, Egypt.
  • Latifa M. Abdelgawad Dental Laser Applications Department, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Egypt.
  • Dalia Y. Ibrahim Restorative and Dental Materials Department, Oral and Dental Research Institute , National Research Centre, Cairo, Egypt.
  • Somaia A. Eissa Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
  • Nada Omar Restorative and Dental Materials Department, Oral and Dental Research Institute , National Research Centre, Cairo, Egypt.: Associate Professor of Endodontics, New Giza University, Giza, Egypt.

Keywords:

Diode laser, Er-YAG laser, Enterococcus faecalis, activated irrigant solutions

Abstract

Background: Enterococcus faecalis is a robust microorganism possessing a diverse array of virulence elements, including lytic enzymes, aggregation substance and lipoteichoic acid. It can also withstand harsh conditions, resist intracanal medicaments, and survive the hard conditions present in root-canal treated teeth. Aim of the work: To assess the impact of 980 nm diode laser photo-activated irrigation and photon-induced photoacoustic streaming (PIPS) - 2940 nanometer Erbium-YAG laser on antibacterial efficacy against the biofilm of Enterococcus faecalis. Methods: Forty-five, single canaled teeth were used. The teeth were evenly distributed among three experimental groups according the final irrigation protocol to: Group I (lateral-vented needle) (n=15), Group II (Diode
laser 980nm) (n=15) (Continuous mode with power 2W) and Group III (Er-YAG laser 2940nm) (n=15) (Pulsed SSP mode, 20mJ, 15Hz and 0.3W). The quantity of irrigant was 4ml NaOCl and activated for 40 seconds in different groups. Each group was split evenly into two subgroups according to the evaluation method: (A) Colony Forming Units (n = 7) and (B) Confocal Laser Scanning Microscope (n
= 7). Results: CFUs results showed that both diode and Er:YAG lasers significantly reduced bacterial counts (97.87%) and (99.79%) respectively than lateral vented needle group (95.21%) with statistically significant difference. Additionally, a significant statistical difference was observed between the Er:YAG and diode laser irrigation activation groups. Conclusion: CLSM results demonstrated a significant statistical variation in CLSM showed in bacterial eradication between groups where it was found in (Er-YAG) (93.75%) followed by (Diode) (77.05%) and (Side vented needle) (10.79%). The best results for laser activation were observed with PIPS, which
resulted in superior disinfection with a higher bacterial reduction. The diode laser group demonstrated the next highest level of bacterial reduction. In contrast, the lateral-vented needle group demonstrated the lowest level of bacterial reduction among all groups. 

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Published

2024-10-25

Issue

Vol. 13 No. 04 (2024): OCTOBER-DECEMBER 2024

Section

Articles

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