Evaluation of the Efficiency of the Chitosan Nanoparticles for Immobilization and Optimization of α-Amylase Produced by Aspergillus oryzae F-923

Authors

  • Nagwa I. Omar Biochemistry Department, National Research Centre, National Research Centre, ElBuhouth Street, P.O. 12622, Giza, Egypt.
  • Shimaa S. Hanafy Biochemistry Department, National Research Centre, National Research Centre, ElBuhouth Street, P.O. 12622, Giza, Egypt.
  • Amr A. El-Ella Department of Measurements Photochemistry and Agriculture Applications. National Institute of Laser Enhanced Science' Cairo University 'Giza' Egypt.
  • M. Fadel Microbial Chemistry Department, National Research Centre, National Research Centre, ElBuhouth Street, P.O. 12622, Giza, Egypt.

Keywords:

Chitosan nanoparticles, Immobilization, α-amylase, A. oryzae F-923

Abstract

In this study, a partially purified α-amylase enzyme, was successfully produced by A. oryzae F-923 and immobilized with chitosan nanoparticles (Cs/GA-NPs). Various concentrations of chitosan nanoparticles were used for immobilization of α amylase enzyme. The highest immobilization yield was for 3 mg/ml concentration with 79.23%. The immobilized amylase enzyme and chitosan nanoparticles (Cs/GA-NPs) are assessed for quality using a number of factors, including native electrophoretic patterns, dynamic light scattering, zeta potential, and UV spectrum. For both free and immobilized α-amylase, the optimum pH levels were 6.5 and 5.5, respectively. The optimum reaction temperature for the immobilized amylase was at 45oC, but it was optimum at 40oC for free one. The Km values for the immobilized and free amylases were 3.5 and 1.7 mg/reaction mixture, respectively. The Vmax for the immobilized and free amylases were 1.25 and 4.76 U/mg, respectively. 

Downloads

Published

2024-09-25

Issue

Vol. 13 No. 03 (2024): JULY-SEPTEMBER 2024

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.