Comparative Histological Study on Anti-Ageing Effect of Hyaluronic Acid Versus Nanofat Injection on the Epidermis of Thin Skin of Female Albino Rat

Abstract

Introduction: Intrinsic skin ageing is an inevitable and physiological process that is characterized by excess wrinkles. Hyaluronic acid (HA) filler is widely used as a dermal filler while nanofat represents a new technique for skin rejuvenation. Aim of the work: This work was carried out to compare between the effect of HA versus nanofat subcutaneous (S.C) injection on the epidermis of aged thin
skin of female albino rats. Materials and Methods: 40 female albino rats were divided into four equal groups. Group I was the control adult one. Group II aged rats were subdivided equally into; subgroup IIa for preparation of the nanofat and subgroup IIb that received a single SC injection of 0.1ml of normal saline at the right flank region. Group III aged rats received a single SC injection of 0.1ml of cross-linked HA at the right flank region. Group IV aged rats received a single SC injection of 0.1ml of nanofat at the same site as group II&III. Skin specimens were examined by LM after their staining with H&E. Morphometric study and statistical analysis were performed to measure the mean total epidermal thickness. Results: LM examination of thin skin of group III and IV showed structural
rejuvenation of their aged epidermis. Morphometric analysis displayed a significant increase in the mean total thickness of the epidermis in thin skin of both group III&IV compared to group II. Conclusion: From the present study, it could be concluded that both hyaluronic acid and nanofat subcutaneous injection have anti-ageing effect on the epidermis of thin skin of female albino rat.