Antimicrobial Activity of Titanium Dioxide Nanoparticles Biosynthesized by Enterococcus hirae


  • Nanis G. Allam Microbiology Unit, Botany Department Faculty of Science, Tanta University, Egypt.
  • Mohammed M. Gharieb Botany and Microbiology Department, Faculty of Science, Menoufia University, Egypt.
  • Noha S. El-Nahrawy Microbiology Unit, Botany Department Faculty of Science, Tanta University, Egypt.


Biological synthesis, Titanium dioxide nanoparticles, Enterococcus hirae, Antimicrobial activity


The present study included the biological synthesis of titanium dioxide nanoparticles by lactic acid bacteria (Enterococcus hirae ATCC 9790). The biosynthesized nanoparticles have been characterized by using ultraviolet visible (UV- vis) spectroscopy, Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD). The UV- Vis spectrum
showed a well-defined surface plasmon resonance peak (SPR) at 326nm.TEMfigure showed pure nanoparticles with mean average size 24.9 nm. FTIR spectroscopy confirmed the presence of biocomponents which were responsible for the synthesis of TiO2NPs.The XRD pattern showed main peak of 2θ=27.4° matches the (110) crystallographic plane of rutile of TiO2 nanoparticles. The biosynthesized TiO2NPs have high antimicrobial activity against pathogenic Gram positive bacteria (Staphylococcus aureus and Bacillus cereus), Gram negative bacteria (Salmonella) and fungi (Penecillium spp.) that causing food poisoning. Results of the present study recommended green synthesis of TiO2NPs by Enterococcus hirae that would play an important role in future concerning industrial, therapeutic and food technology applications.